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4 Circadian rhythms regulate post-IS BBB repair via immune system modulation 4.1 Circadian rhythms orchestrate immune cell-mediated BBB repair following IS After stroke, BBB dysfunction involves several inflammatory mechanisms, including increased matrix metalloproteinases (MMP-2/9), excessive ROS production, polarization of microglia toward pro-inflammatory states, and infiltration of peripheral leukocytes (8891)